Reliability and comparison of the polymorphism revealed in amaranth by amplified fragment length polymorphisms (AFLPs) and inters simple sequence repeats (ISSRs)

Genetic diversity and relationships among Chinese Eucommia ulmoides cultivars revealed by sequence-related amplified polymorphism, amplified fragment length polymorphism, and inter-simple sequence repeat markers.

Sequence-related amplified polymorphism (SRAP), amplified fragment length polymorphism (AFLP), and inter-simple sequence repeat (ISSR) markers were used to estimate the genetic diversity and relationships among Eucommia ulmoides cultivars in China. A total of 240, 192, and 150 DNA fragments were detected by 10 SRAP primer combinations, 10 AFLP primer combinations, and 10 ISSR primers, among whi...

Individual and population variation in invertebrates revealed by Inter-simple Sequence Repeats (ISSRs)

PCR-based molecular markers are well suited for questions requiring large scale surveys of plant and animal populations. Inter-simple Sequence Repeats or ISSRs are analyzed by a recently developed technique based on the amplification of the regions between inverse-oriented microsatellite loci with oligonucleotides anchored in microsatellites themselves. ISSRs have shown much promise for the stu...

Genetic Polymorphism between Tobacco Cultivar-groups Revealed by Amplified Fragment Length Polymorphism Analysis

DNA variation in the wild plant Arabidopsis thaliana revealed by amplified fragment length polymorphism analysis.

To investigate the level and pattern of DNA variation of Arabidopsis thaliana at the entire genome level, AFLP analysis was conducted for 38 ecotypes distributed throughout the world. Ten pairs of selective primers were used to detect a total of 472 bands, of which 374 (79. 2%) were polymorphic. The frequency distribution of polymorphic bands was skewed toward an excess of singleton variation. ...

Taxonomic status of the Davis Mountains cottontail, Sylvilagus robustus, revealed by amplified fragment length polymorphism

The Davis Mountains cottontail, Sylvilagus robustus, is morphologically different from the eastern cottontail, S. floridanus, but previous genetic analysis of mitochondrial DNA data did not recover 2 genetically distinct groups. Our study used a nuclear DNA fingerprinting technique, amplified fragment length polymorphism (AFLP), to test the hypothesis that S. robustus is genetically distinct fr...